Simplify intact phosphoprotein analysis with on-line separation and analysis by CESI-MS
Dr. Stephen Lock
Marketing Development Manager, SCIEX
Performing on-line, high-resolution separation of large intact molecule proteoforms prior to mass spectrometry (MS) analysis is virtually impossible. In order to come close, one must either implement an immunocapture enrichment strategy and/or lengthy cation exchange, pre-fractionation steps prior to MS analysis. In this webinar, we demonstrate the use of one CESI-MS method for high-resolution separation of intact proteoforms that could save you lab hours of preparation work.
We will use protein standards to demonstrate how CESI-MS separations of intact proteins differ from separations obtained by standard reverse phase LC-MS analysis. We will also show data from alpha casein which has been used to compare CESI-MS with LC-MS for the separation of intact phosphoproteins.
Key Learning Objectives:
- Why CESI-MS is ideal for intact protein analysis
- How CESI-MS compares with LC-MS for intact protein analysis
- How CESI-MS can be used to get high resolution separation of intact phosphoproteins based on their charge
Comments | 0 ADD COMMENT
Like what you just read? You can find similar content on the communities below.Analysis & Separations Applied Sciences Biopharma Cancer Research Diagnostics Drug Discovery Genomics Research Neuroscience
To personalize the content you see on Technology Networks homepage, Log In or Subscribe for FreeLOGIN SUBSCRIBE FOR FREE