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Strategies to compare far-UV Circular Dichroism spectra of similar proteins using Chirascan™-plus Automated Circular Dichroism

Due to the very low sample volumes required and the simplicity of the measurement, it is a powerful comparative tool to detect differences in structure between proteins. Here, Applied Photophysics explores two ways of analysing CD spectra taken in the far ultraviolet, that do not require quantitation of the protein concentration.