Using a Scientifica Multiphoton SliceScope for Fluorescence Lifetime Imaging
White Paper Feb 16, 2017
2-P FLIM-FRET system schematic. Scientifica
In this white paper, the authors describe the importance of Fluorescence Lifetime IMaging (FLIM) and Fluorescence Resonance Energy Transfer (FRET) techniques in neuroscience.
By reading this white paper you will:
- Gain an understanding of the basic principles behind the techniques.
- Understand how to implement FLIM and FRET techniques in your experiments.
- Read about an example experiment using FLIM-FRET to monitor conformational changes in the NMDA receptors in response to agonist binding, in the spines of hippocampal neurons, using 2-photon imaging technologies.