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Latest App Notes & Case Studies

App Note / Case Study

Rapid and Reproducible DNA Isolation from 1 ml of Whole Blood with Thermo Scientific KingFisher Flex

This application note shows the benefits of KingFisher Flex by using genomic DNA isolation from blood as an example.
App Note / Case Study

Reproducible Dispensing of Live Cells with the Thermo Scientific Multidrop Combi Reagent Dispenser

High throughput cell-based assays are an important part of drug screening, and successfully dispensing viable cells is a key step in the process. Automated reagent dispensers are used to achieve higher throughput in the screening laboratories. Dispense must not only be quick, it must also be gentle enough to ensure cell viability.
App Note / Case Study

Device Qualification of the Thermo Scientific Finnpipette Novus Electronic Pipette (IQ/OQ/PQ)

During device qualification, one verifies and documents whether the equipment, in this case a pipette, is reliable and capable of fulfilling the requirements of its intended use. All qualification activities are fully documented according to pertinent quality and regulatory requirements and signed by authorized personnel.
App Note / Case Study

Use of Thermo Scientific Finnpipette Novus Electronic Pipettes for Accurate and Efficient Preparation of Standard Curves

This application note discusses the use of a Thermo Scientific Finnpipette Novus Electronic Pipette for the preparation of standard curves. With 10 different functions, the versatile Novus Electronic pipette can perform most every standard curve required. The pipette’s performance was compared to a manual Thermo Scientific Finnpipette model to determine if there were appreciable differences in pipetting speed and accuracy during curve preparation.
App Note / Case Study

Validated Luminometric DLR™ Reporter Gene Assay with Thermo Scientific Varioskan® Flash

One of the most common luminometric assays is Dual Luciferase® Reporter Assay from Promega corp. This application note shows how this assay is performed with Varioskan Flash multitechnology microplate reader. The note includes the performance of the normal DLR assay and results from some tests showing important aspects of the basic behaviour and performance of the DLR™ chemistry. This paper describes the overall efficiency of the Varioskan Flash in the measurement of DLR assay.
App Note / Case Study

Characterization of Emission Properties of Aequorin Mutants with Rapid, High Throughput Screening Assay

This application note explains how to perform a rapid, high throughput screening assay of mutated photoprotein aequorin in order to find clones with more intense, slower kinetic, narrow spectral or color-shifted bioluminescent emission. Several examples of bioluminescent emission kinetics and spectra of mutated aequorins are included.
App Note / Case Study

Optimization of the DNA Purification Protocol for the Thermo Scientific KingFisher Flex and BindIt 3.1 Software

Purpose: Optimal quantity and quality of DNA with the magnetic particle based extraction technology. Method: Thermo Scientific KingFisher Flex magnetic particle processor and BindIt® 3.1 Software. Results: By simple optimization of the nucleic acid extraction protocol it is possible to achieve a great yield of high quality DNA.
App Note / Case Study

Potassium Bromate in Bakery Products

Potassium bromate is recognised as one of the best dough conditioners in the bakery industry. Scientific evidence surfaced in 1990, implicating bromate as a possible carcinogen. In response the United Nations Joint Food and Agricultural Organisation and the WHO reduced bromate residuals to less than 5 parts per billion (ppb) in finished products.
App Note / Case Study

Simultaneous Analysis of Anions and Cations by Tandem Chromatography with Conductivity Detection

Tandem chromatography is emerging as a powerful technique to double the output of HPLC equipment by simultaneously carrying out two different analyses on the same sample. The principle is straightforward: the sample is split in two at the injection port, and each aliquot is eluted on a different column and analysed by a different detector.
App Note / Case Study

Determination of Sudan I and its Analogues in food

Sudan I is the common name given to 1-phenylazo-2-naphthol, a fat-soluble dye used industrially to colour oils, waxes, greases, varnishes etc. It has been recognised by health authorities as being potentially carcinogenic, and is banned as a food additive in the UK and across the European Union.
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