Latest App Notes & Case Studies

During manufacture of antibody molecules, they are subjected to mechanical stress generated by processes such as pumping and filtration. This may cause denaturation and consequently aggregation due to exposure of the protein to air-liquid interfaces and shear forces, resulting in the ultimate loss in bioactivity.

G-protein coupled receptors (GPCRs) are a large family of receptors which have a broad involvement in cellular responses affecting many important body functions both in health and disease.

Pgp expression has been linked to the effl ux of chemotherapeutic
drugs in human cancers leading to multidrug resistance. Pgp
activity can also result in low oral absorption and poor brain
penetration. Interaction of drugs with the Pgp may also cause
an increase in toxicity of co-administered compounds.

Numerous studies, employing a wide variety of methods, have been carried out to determine the typical caffeine content of commonly consumed beverages. This application note details how UV spectroscopy can be used to analyse and quantify the caffeine content of some common beverages and soda drinks.

The aspirin content of commercial tablets is routinely determined using the acid/base titration method. This application note details the UV and Visible spectrophotometric methods for determining the aspirin content of tablets.

The use of the Glucose (HK) Assay Kit from Sigma can be used to measure the amount of glucose in three different types of drink.

Common applications of UV/Visible spectrophotometers include direct measurement of nucleic acids and proteins to determine their concentration. Using Jenway UV plastic cuvettes with a microcuvette holder, these measurements are possible with as little as 70µl of sample.

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of the FLUOstar OPTIMA multidetection microplate reader from BMG LABTECH.

Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.

LanthaScreen technology uses time-resolved fluorescence resonance energy transfer (TR-FRET) to study multiple target classes including protein kinases, nuclear hormone receptors, as well as proteases and ubiquitinated proteins.