Latest App Notes & Case Studies
App Note / Case Study
Quantitative Determination of Caffeine in Beverages and soft Drinks Using UV Wavelength Spectroscopy
Numerous studies, employing a wide variety of methods, have been carried out to determine the typical caffeine content of commonly consumed beverages. This application note details how UV spectroscopy can be used to analyse and quantify the caffeine content of some common beverages and soda drinks.
App Note / Case Study
Quantitative Determination of the Aspirin Content of Tablets Using UV and Visible Wavelength Spectroscopy
The aspirin content of commercial tablets is routinely determined using the acid/base titration method. This application note details the UV and Visible spectrophotometric methods for determining the aspirin content of tablets.
App Note / Case Study
Quantitative Determination of Glucose in an Energy Drink, Apple Juice and Wine
The use of the Glucose (HK) Assay Kit from Sigma can be used to measure the amount of glucose in three different types of drink.
App Note / Case Study
Use of the Microcuvette Holder with Plastic Microcuvettes
Common applications of UV/Visible spectrophotometers include direct measurement of nucleic acids and proteins to determine their concentration. Using Jenway UV plastic cuvettes with a microcuvette holder, these measurements are possible with as little as 70µl of sample.
App Note / Case Study
Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA Microplate Reader
The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of the FLUOstar OPTIMA multidetection microplate reader from BMG LABTECH.
App Note / Case Study
Quantitative Determination of Total Bile Acids with a Thermo Scientific Varioskan Flash Multimode Reader Using a Kinetic Enzyme Cycling Assay
Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.
App Note / Case Study
LanthaScreen TR-FRET Assay Performance with Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader
LanthaScreen technology uses time-resolved fluorescence resonance energy transfer (TR-FRET) to study multiple target classes including protein kinases, nuclear hormone receptors, as well as proteases and ubiquitinated proteins.
App Note / Case Study
Optimization of Instrument Settings and Measurement Parameters for Kinetic Fluorometric and Luminometric Assays
When fluorometric and luminometric assays are performed in microplate format, a high number of samples can be analyzed at a time. The speed of the reaction kinetics of the assay determines the requirements for the detection instrument and measurement parameters for achieving the best possible performance of the assay.
App Note / Case Study
Pipetting performance of Thermo Scientific Finnpipette F2 in combination with Thermo Scientific Finntip Flex
The Finnpipette® F2 product family has been designed by taking into account the demands of today´s laboratory work and the require¬ments of the end users. It offers superior comfort, performance, reliability and repeatability in one of the lightest pipettes available.
App Note / Case Study
Turbidometric Growth Curve Measurements and Liquid Evaporation Studies with a Microplate Photometer
The turbidity of a liquid sample is traditionally measured either with a nephelometer or a photometer. The measure of turbidity (also called optical density, OD) refers to the scattering of light from the particles of the measured sample. When measuring turbidity of a bacterial culture, the amount of scattered light is directly proportional to the number of bacterial cells in the sample.
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