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Application Notes

 
General V3 Western Blotting Protocol
Application Note

Bio-Rad Laboratories

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.

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Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™
Application Note

Bio-Rad Laboratories

Traditional western blot workflows require an appropriate sample load to detect both target proteins and loading control proteins. The discrepancy between these quantities often leads to oversaturated protein bands, forcing researchers to sometimes run two duplicate gels or titrate down the antibody concentration in order to get quantitative results.

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Determining the Appropriate Sample Load for Western Blots
Application Note

Bio-Rad Laboratories

Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak signals.

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Avoiding Housekeeping Protein Detection Saturation
Application Note

Bio-Rad Laboratories

Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.

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Improved cell viability assay performance using PrestoBlue® Reagent
Application Note

Cell proliferation and viability assays are widely used in academic and life science laboratories, and are becoming increasingly common in the pharmaceutical industry. Assessment of cellular viability markers in cell-based applications is mandatory for laboratories working with in vitro systems.

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Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products
Application Note

BioTek Instruments Inc.

Several challenges have surfaced during clinical evaluation of biological drug products due to a commonly associated immune response in patients. Anti-drug antibodies (ADA) are known to be frequently generated during administration of humanized monoclonal antibody therapeutics. A commonly used technology platform for assessment of immunogenicity relies on the bridging immunogenicity assay format typical of the ELISA.

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Molecular indexing for improved RNA-Seq analysis
Application Note

Bioo Scientific

Most modern methods for nucleic acid analysis require the use of enzyme processing, such as DNA polymerase reactions, in the sample preparation or measurement steps. Although necessary, these enzymatic steps introduce errors in the form of incorrect sequence and misrepresented copy number. NEXTflex™ qRNA-Seq™ Kit from Bioo Scientific adds no additional steps to the work flow, costs no more than a conventional library preparation kit and increases the precision of downstream analysis.

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Determination of sodium in margarine manufacture
Application Note

Metrohm AG

This application note describes the determination of the total sodium content of precursor solutions used in margarine manufacture.

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Determination of sodium in soy milk
Application Note

Metrohm AG

This Application Note describes the determination of the total Sodium content in soy milk products. The methodology may also be applied to the determination of Sodium in milk from cows, goats, and sheep.

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An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture
Application Note

Bioo Scientific

The studies described herein demonstrate the capability of Bioo Scientific’s novel NEXTFlex™ DNA Pre-Capture Combo Library construction kits used in conjunction with the NimbleGen Sequence Capture technology to obtain high coverage comparative genomic data from a panel of human HapMap DNA samples.

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