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Application Notes

 
Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
Application Note

Molecular Devices

Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.

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Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Application Note

Molecular Devices

The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.

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Recording of ICRAC in RBL-1 Cells on the PatchXpress 7000A Automated Patch Clamp System
Application Note

Molecular Devices

Calcium release-activated calcium (CRAC) channels play an important role in intracellular Ca2+ homeostasis. Like other store-operated calcium channels, currents through the CRAC channel (ICRAC) are activated by depletion of calcium in the endoplasmic reticulum (ER) and serve the purpose of slowly replenishing the ER with Ca2+.

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Optimizing the Labeling of Proteins
Application Note

Molecular Devices

The Threshold® Immuno-Ligand Assay (ILA) adapts reagents used in ELISA, RIA or radioreceptor assays for the Threshold System by covalently attaching biotin or fluorescein labels to the binding proteins and/or ligands. To simplify labeling and purification of proteins, Molecular Devices has formulated biotin and fluorescein as N-hydroxysuccinimide esters.

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Overcoming the Challenge of Reliable Multi-way Fluidic Connections
Application Note

Dolomite

If you are struggling to create fast and reliable microfluidic connections, Multiflux™ may be the solution you are looking for.

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BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay
Application Note

BMG Labtech

The BlueScreen HC assay from Gentronix utilizes a luminescence-based reporter system to measure genotoxicity. The assay generates positive results e.g for direct-acting mutagens and clastogens and correct negative results for non-carcinogens. In addition, reduced cell proliferation, a measure of cytotoxicity, is assessed, too. Using the FLUOstar Omega microplate reader from BMG LABTECH the BlueScreen HC assay has been shown to be fast, accurate and reproducible.

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DNA Measurements in Low Volume Samples, Microplates and Cuvettes on the SPECTROstar Nano
Application Note

BMG Labtech

This application note details how the SPECTROstar Nano absorbance reader measures nucleotides such as DNA in microplates, cuvettes and low volume samples with the BMG LABTECH’s LVis Plate.

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Growth of Campylobacter using BMG LABTECH’s FLUOstar Omega equipped with ACU
Application Note

BMG Labtech

Campylobacter are Gram negative microaerophilic organisms which are a common commensal of mammals and birds and are one of the most frequently isolated causative agents of human bacterial enteritis worldwide. Infection in humans is frequently associated with the consumption of under cooked poultry and is generally self limiting though it can in a small number of cases result in severe complications such as Guillain-Barre syndrome.

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Miniaturised Platelet Aggregation Assays using the NOVOstar Microplate Reader
Application Note

BMG Labtech

Platelets are small, anucleate blood cells derived from their precursor cells, megakaryocytes that are vital for haemostasis. Following blood vessel injury, subendothelial collagens are exposed to which platelets adhere. The detailed characterisation of platelet function and underlying regulatory mechanisms is important for the development of new strategies to selectively reduce platelet reactivity and prevent thrombosis.

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mosquito® Crystal for the rapid screening and optimisation of crystallisation conditions of the bacterial periplasmic binding protein, HbpA
Application Note

Trevigen

Glutathione is one of the major antioxidants/redox molecules in most aerobic cells, playing an important role in cell metabolism, differentiation, proliferation, and apoptosis. The reduced form of glutathione (GSH) is required for the detoxification of drugs and reactive compounds of both endogenous and exogenous origin.

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