We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Application Notes

 
PSVue®: Novel small molecule probes for fluorescent imaging of apoptosis and bacterial infections in vitro and in vivo
Application Note

PSVue® offers a robust alternative to probes currently used by researchers to detect early-to-mid stage apoptosis, as well as bacterial infections.

READ MORE

Determining Total Sugar Content in Maple Syrup to Meet FDA Nutrition Labeling Requirements
Application Note

Agilent Technologies

Nutrition labels on food items provide valuable information to consumers. To ensure authenticity, the Food and Drug Administration checks the reported values on the nutrition labels. Due to advances in chromatography and universal analysis of sugars by refractive index detectors (RI), RI is the preferred method for sugar analysis. A total sugar analysis method for commercially available maple syrup was performed using Agilent HiPlex Ca Column and Agilent 1260 Infinity RI Detector.

READ MORE

Analysis of Acharicides in Honey
Application Note

Agilent Technologies

To prevent economic losses beekeepers treat their colonies with acaricides. The use of these acaricides inside beehives implies a risk of direct pollution of honey and other hive products, therefore maximum residue levels on honey have been fixed in many countries to protect consumers.

READ MORE

Two-Dimensional HPLC Combined with On-Line SPE for Determination of Sudan Dyes I–IV in Chili Oil
Application Note

Dionex - Part of Thermo Fisher Scientific

Although use of Sudan dyes in food is restricted, they are nevertheless sometimes added to foods to improve the appearance and command a higher price. Because Sudan dyes may create health problems such as genotoxic and carcinogenic effects, concerns over contamination of Sudan dyes has increased awareness and testing for these compounds.

READ MORE

Fast Determination of Anthocyanins in Pomegranate Juice
Application Note

Due to their strong antioxidant properties, anthocyanins are of considerable interest. Pomegranate juice (PJ) has been reported to contain 3× more antioxidant activity than green tea. Due to the high demand for pomegranates, adulteration of PJ has become widespread. Manufacturers have attempted to extend the limited supply by blending with filler ingredients such as cane sugar, corn syrup sweeteners, and lower-quality juices containing sorbitol, malic acid, and sucrose.

READ MORE

Analysis of Fruit Juice Adulterated with Medium Invert Sugar from Beets
Application Note

Dionex - Part of Thermo Fisher Scientific

Fruit juice adulteration presents an economic and regulatory problem. The United States orange juice industry estimates that orange juice sales gross more than one billion dollars annually. The most common forms of adulteration include simple dilution and blending of inexpensive and synthetically produced juices into the more expensive ones.

READ MORE

Kv1.5 Potassium Channel Assay Using the IonWorks HT System
Application Note

Molecular Devices

This application note describes the use of the IonWorks HT Platform with a voltagedependent potassium channel Kv1.5.

READ MORE

Contaminant bovine IgG assay
Application Note

Molecular Devices

Fetal calf serum containing bovine IgG is commonly used in mammalian cell culture media for the production of recombinant pharmaceuticals. During the purification process of a recombinant pharmaceutical, bovine IgG may be copurified with the product, and may represent a biological hazard for clinical use. Therefore, it may be necessary to assay for contaminant bovine IgG in a final product originating from mammalian cells.

READ MORE

Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System
Application Note

Molecular Devices

Accurate measurement of the concentration of a biological product is important in bioreactor/fermenter monitoring, downstream processing and final product quality control. Currently, electrophoretic, chromatographic and immunochemical methods are the most commonly used. These three methods are currently used in combination to provide the desired sample information.

READ MORE

Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
Application Note

Molecular Devices

Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.

READ MORE