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Application Notes

 
Two-Dimensional HPLC Combined with On-Line SPE for Determination of Sudan Dyes I–IV in Chili Oil
Application Note

Dionex - Part of Thermo Fisher Scientific

Although use of Sudan dyes in food is restricted, they are nevertheless sometimes added to foods to improve the appearance and command a higher price. Because Sudan dyes may create health problems such as genotoxic and carcinogenic effects, concerns over contamination of Sudan dyes has increased awareness and testing for these compounds.

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Fast Determination of Anthocyanins in Pomegranate Juice
Application Note

Due to their strong antioxidant properties, anthocyanins are of considerable interest. Pomegranate juice (PJ) has been reported to contain 3× more antioxidant activity than green tea. Due to the high demand for pomegranates, adulteration of PJ has become widespread. Manufacturers have attempted to extend the limited supply by blending with filler ingredients such as cane sugar, corn syrup sweeteners, and lower-quality juices containing sorbitol, malic acid, and sucrose.

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Analysis of Fruit Juice Adulterated with Medium Invert Sugar from Beets
Application Note

Dionex - Part of Thermo Fisher Scientific

Fruit juice adulteration presents an economic and regulatory problem. The United States orange juice industry estimates that orange juice sales gross more than one billion dollars annually. The most common forms of adulteration include simple dilution and blending of inexpensive and synthetically produced juices into the more expensive ones.

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Kv1.5 Potassium Channel Assay Using the IonWorks HT System
Application Note

Molecular Devices

This application note describes the use of the IonWorks HT Platform with a voltagedependent potassium channel Kv1.5.

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Contaminant bovine IgG assay
Application Note

Molecular Devices

Fetal calf serum containing bovine IgG is commonly used in mammalian cell culture media for the production of recombinant pharmaceuticals. During the purification process of a recombinant pharmaceutical, bovine IgG may be copurified with the product, and may represent a biological hazard for clinical use. Therefore, it may be necessary to assay for contaminant bovine IgG in a final product originating from mammalian cells.

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Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System
Application Note

Molecular Devices

Accurate measurement of the concentration of a biological product is important in bioreactor/fermenter monitoring, downstream processing and final product quality control. Currently, electrophoretic, chromatographic and immunochemical methods are the most commonly used. These three methods are currently used in combination to provide the desired sample information.

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Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
Application Note

Molecular Devices

Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.

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Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Application Note

Molecular Devices

The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.

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Recording of ICRAC in RBL-1 Cells on the PatchXpress 7000A Automated Patch Clamp System
Application Note

Molecular Devices

Calcium release-activated calcium (CRAC) channels play an important role in intracellular Ca2+ homeostasis. Like other store-operated calcium channels, currents through the CRAC channel (ICRAC) are activated by depletion of calcium in the endoplasmic reticulum (ER) and serve the purpose of slowly replenishing the ER with Ca2+.

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Optimizing the Labeling of Proteins
Application Note

Molecular Devices

The Threshold® Immuno-Ligand Assay (ILA) adapts reagents used in ELISA, RIA or radioreceptor assays for the Threshold System by covalently attaching biotin or fluorescein labels to the binding proteins and/or ligands. To simplify labeling and purification of proteins, Molecular Devices has formulated biotin and fluorescein as N-hydroxysuccinimide esters.

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