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Application Notes

 
Use of the Microcuvette Holder with Plastic Microcuvettes
Application Note

Jenway

Common applications of UV/Visible spectrophotometers include direct measurement of nucleic acids and proteins to determine their concentration. Using Jenway UV plastic cuvettes with a microcuvette holder, these measurements are possible with as little as 70µl of sample.

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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA Microplate Reader
Application Note

BMG Labtech

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of the FLUOstar OPTIMA multidetection microplate reader from BMG LABTECH.

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Quantitative Determination of Total Bile Acids with a Thermo Scientific Varioskan Flash Multimode Reader Using a Kinetic Enzyme Cycling Assay
Application Note

Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.

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LanthaScreen TR-FRET Assay Performance with Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader
Application Note

LanthaScreen technology uses time-resolved fluorescence resonance energy transfer (TR-FRET) to study multiple target classes including protein kinases, nuclear hormone receptors, as well as proteases and ubiquitinated proteins.

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Optimization of Instrument Settings and Measurement Parameters for Kinetic Fluorometric and Luminometric Assays
Application Note

When fluorometric and luminometric assays are performed in microplate format, a high number of samples can be analyzed at a time. The speed of the reaction kinetics of the assay determines the requirements for the detection instrument and measurement parameters for achieving the best possible performance of the assay.

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Pipetting performance of Thermo Scientific Finnpipette F2 in combination with Thermo Scientific Finntip Flex
Application Note

The Finnpipette® F2 product family has been designed by taking into account the demands of today´s laboratory work and the require¬ments of the end users. It offers superior comfort, performance, reliability and repeatability in one of the lightest pipettes available.

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Turbidometric Growth Curve Measurements and Liquid Evaporation Studies with a Microplate Photometer
Application Note

The turbidity of a liquid sample is traditionally measured either with a nephelometer or a photometer. The measure of turbidity (also called optical density, OD) refers to the scattering of light from the particles of the measured sample. When measuring turbidity of a bacterial culture, the amount of scattered light is directly proportional to the number of bacterial cells in the sample.

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An Easy Determination of the Antioxidant Power of Beverage Samples with Thermo Scientific Multiskan FC Microplate Photometer
Application Note

Oxidative stress has been demonstrated to be involved in many diseases including atherochlerosis, Parkinson disease and cancer. Oxidative stress is caused by an imbalance between the production of reactive oxygen and a biological system’s ability to detoxify the reactive intermediates, or easily repair the resulting damages.

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High Sensitivity ELISA Assays for the Detection of Melamine Residuals in Milk
Application Note

Melamine is an industrial chemical most commonly used as a fire retardant in dry powder extinguishers due to its release of nitrogen gas when burned or charred. Melamine has been used illegally by adding it to food products in order to increase the apparent protein content.

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Facilitating Biofilms Assays with Thermo Scientific Multidrop® Combi Dispenser
Application Note

In recent years, the automation of cell (including bacteria)-based screening assays has consistently shown to provide reduced times and considerable labor saving, while maintaining or increasing assay quality during screening campaigns.

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