We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Application Notes

 
Reproducible Dispensing of Live Cells with the Thermo Scientific Multidrop Combi Reagent Dispenser
Application Note

High throughput cell-based assays are an important part of drug screening, and successfully dispensing viable cells is a key step in the process. Automated reagent dispensers are used to achieve higher throughput in the screening laboratories. Dispense must not only be quick, it must also be gentle enough to ensure cell viability.

READ MORE

Device Qualification of the Thermo Scientific Finnpipette Novus Electronic Pipette (IQ/OQ/PQ)
Application Note

During device qualification, one verifies and documents whether the equipment, in this case a pipette, is reliable and capable of fulfilling the requirements of its intended use. All qualification activities are fully documented according to pertinent quality and regulatory requirements and signed by authorized personnel.

READ MORE

Use of Thermo Scientific Finnpipette Novus Electronic Pipettes for Accurate and Efficient Preparation of Standard Curves
Application Note

This application note discusses the use of a Thermo Scientific Finnpipette Novus Electronic Pipette for the preparation of standard curves. With 10 different functions, the versatile Novus Electronic pipette can perform most every standard curve required. The pipette’s performance was compared to a manual Thermo Scientific Finnpipette model to determine if there were appreciable differences in pipetting speed and accuracy during curve preparation.

READ MORE

Validated Luminometric DLR™ Reporter Gene Assay with Thermo Scientific Varioskan® Flash
Application Note

One of the most common luminometric assays is Dual Luciferase® Reporter Assay from Promega corp. This application note shows how this assay is performed with Varioskan Flash multitechnology microplate reader. The note includes the performance of the normal DLR assay and results from some tests showing important aspects of the basic behaviour and performance of the DLR™ chemistry. This paper describes the overall efficiency of the Varioskan Flash in the measurement of DLR assay.

READ MORE

Characterization of Emission Properties of Aequorin Mutants with Rapid, High Throughput Screening Assay
Application Note

This application note explains how to perform a rapid, high throughput screening assay of mutated photoprotein aequorin in order to find clones with more intense, slower kinetic, narrow spectral or color-shifted bioluminescent emission. Several examples of bioluminescent emission kinetics and spectra of mutated aequorins are included.

READ MORE

Optimization of the DNA Purification Protocol for the Thermo Scientific KingFisher Flex and BindIt 3.1 Software
Application Note

Purpose: Optimal quantity and quality of DNA with the magnetic particle based extraction technology. Method: Thermo Scientific KingFisher Flex magnetic particle processor and BindIt® 3.1 Software. Results: By simple optimization of the nucleic acid extraction protocol it is possible to achieve a great yield of high quality DNA.

READ MORE

Potassium Bromate in Bakery Products
Application Note

Cecil Instruments Ltd

Potassium bromate is recognised as one of the best dough conditioners in the bakery industry. Scientific evidence surfaced in 1990, implicating bromate as a possible carcinogen. In response the United Nations Joint Food and Agricultural Organisation and the WHO reduced bromate residuals to less than 5 parts per billion (ppb) in finished products.

READ MORE

Simultaneous Analysis of Anions and Cations by Tandem Chromatography with Conductivity Detection
Application Note

Cecil Instruments Ltd

Tandem chromatography is emerging as a powerful technique to double the output of HPLC equipment by simultaneously carrying out two different analyses on the same sample. The principle is straightforward: the sample is split in two at the injection port, and each aliquot is eluted on a different column and analysed by a different detector.

READ MORE

Determination of Sudan I and its Analogues in food
Application Note

Cecil Instruments Ltd

Sudan I is the common name given to 1-phenylazo-2-naphthol, a fat-soluble dye used industrially to colour oils, waxes, greases, varnishes etc. It has been recognised by health authorities as being potentially carcinogenic, and is banned as a food additive in the UK and across the European Union.

READ MORE

Analysis of Inorganic Cations in Drinking and Waste Water by Cation Exchange Chromatography with Conductivity Detection
Application Note

Cecil Instruments Ltd

Inorganic cations are significant indicators of the quality of drinking waters and their suitability for human consumption. They have important physiological effects and they alter the palatability of water.

READ MORE