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Application Notes

 
Pathway independent cell-based assays on the EnSpire Multimode Plate Reader with Epic® label-free technology
Application Note

PerkinElmer

This application note details how the PerkinElmer EnSpire® Multimode Plate Reader with Corning® Epic® label-free technology can be used in multi-well microplate formats to non-invasively identify and characterize multiple G protein-coupled receptor (GPCR) pathways in living cells using an orthogonal approach.

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Comparison of Performance: EnSpire Multimode Plate Reader and Corning® Epic® System
Application Note

PerkinElmer

In recent years, label-free technology has gained momentum as an innovative tool in the fields of cellular research and biochemical binding assays. This application note demonstrates the comparable performance of the EnSpire Multimode Plate Reader to the Corning® Epic® System.

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Quantitative Determination of Caffeine in Beverages and soft Drinks Using UV Wavelength Spectroscopy
Application Note

Jenway

Numerous studies, employing a wide variety of methods, have been carried out to determine the typical caffeine content of commonly consumed beverages. This application note details how UV spectroscopy can be used to analyse and quantify the caffeine content of some common beverages and soda drinks.

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Quantitative Determination of the Aspirin Content of Tablets Using UV and Visible Wavelength Spectroscopy
Application Note

Jenway

The aspirin content of commercial tablets is routinely determined using the acid/base titration method. This application note details the UV and Visible spectrophotometric methods for determining the aspirin content of tablets.

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Quantitative Determination of Glucose in an Energy Drink, Apple Juice and Wine
Application Note

Jenway

The use of the Glucose (HK) Assay Kit from Sigma can be used to measure the amount of glucose in three different types of drink.

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Use of the Microcuvette Holder with Plastic Microcuvettes
Application Note

Jenway

Common applications of UV/Visible spectrophotometers include direct measurement of nucleic acids and proteins to determine their concentration. Using Jenway UV plastic cuvettes with a microcuvette holder, these measurements are possible with as little as 70µl of sample.

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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA Microplate Reader
Application Note

BMG Labtech

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of the FLUOstar OPTIMA multidetection microplate reader from BMG LABTECH.

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Quantitative Determination of Total Bile Acids with a Thermo Scientific Varioskan Flash Multimode Reader Using a Kinetic Enzyme Cycling Assay
Application Note

Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.

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LanthaScreen TR-FRET Assay Performance with Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader
Application Note

LanthaScreen technology uses time-resolved fluorescence resonance energy transfer (TR-FRET) to study multiple target classes including protein kinases, nuclear hormone receptors, as well as proteases and ubiquitinated proteins.

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Optimization of Instrument Settings and Measurement Parameters for Kinetic Fluorometric and Luminometric Assays
Application Note

When fluorometric and luminometric assays are performed in microplate format, a high number of samples can be analyzed at a time. The speed of the reaction kinetics of the assay determines the requirements for the detection instrument and measurement parameters for achieving the best possible performance of the assay.

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