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Application Notes

 
Dispense Liquids Containing Proteins More Reliably with Reverse Pipetting
Application Note

This application note demonstrates how the reverse pipetting technique can improve the repeatability of dispensing liquids that contain proteins.

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DNA Quantitation in Cuvette and TrayCell using the Thermo Scientific Multiskan GO Spectrophotometer
Application Note

This application note describes how to perform photometric DNA quantitation and qualification measurements with the Thermo Scientific Multiskan GO microplate and cuvette spectrophotometer in various cuvette formats.

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Antimicrobial Properties of Thermo Scientific Finnpipette F1 Compared to Traditional Pipettes
Application Note

In this application note the antimicrobial properties of the Thermo Scientific Finnpipette F1 were compared to traditional pipettes without antimicrobial treatment. The experiments were performed by an independent laboratory (Institut für Biotechnische Forschung und Entwicklung Graf GmbH, Kirkel-Limbach, Germany).

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Rapid and Reproducible DNA Isolation from 1 ml of Whole Blood with Thermo Scientific KingFisher Flex
Application Note

This application note shows the benefits of KingFisher Flex by using genomic DNA isolation from blood as an example.

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Reproducible Dispensing of Live Cells with the Thermo Scientific Multidrop Combi Reagent Dispenser
Application Note

High throughput cell-based assays are an important part of drug screening, and successfully dispensing viable cells is a key step in the process. Automated reagent dispensers are used to achieve higher throughput in the screening laboratories. Dispense must not only be quick, it must also be gentle enough to ensure cell viability.

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Device Qualification of the Thermo Scientific Finnpipette Novus Electronic Pipette (IQ/OQ/PQ)
Application Note

During device qualification, one verifies and documents whether the equipment, in this case a pipette, is reliable and capable of fulfilling the requirements of its intended use. All qualification activities are fully documented according to pertinent quality and regulatory requirements and signed by authorized personnel.

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Use of Thermo Scientific Finnpipette Novus Electronic Pipettes for Accurate and Efficient Preparation of Standard Curves
Application Note

This application note discusses the use of a Thermo Scientific Finnpipette Novus Electronic Pipette for the preparation of standard curves. With 10 different functions, the versatile Novus Electronic pipette can perform most every standard curve required. The pipette’s performance was compared to a manual Thermo Scientific Finnpipette model to determine if there were appreciable differences in pipetting speed and accuracy during curve preparation.

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Validated Luminometric DLR™ Reporter Gene Assay with Thermo Scientific Varioskan® Flash
Application Note

One of the most common luminometric assays is Dual Luciferase® Reporter Assay from Promega corp. This application note shows how this assay is performed with Varioskan Flash multitechnology microplate reader. The note includes the performance of the normal DLR assay and results from some tests showing important aspects of the basic behaviour and performance of the DLR™ chemistry. This paper describes the overall efficiency of the Varioskan Flash in the measurement of DLR assay.

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Characterization of Emission Properties of Aequorin Mutants with Rapid, High Throughput Screening Assay
Application Note

This application note explains how to perform a rapid, high throughput screening assay of mutated photoprotein aequorin in order to find clones with more intense, slower kinetic, narrow spectral or color-shifted bioluminescent emission. Several examples of bioluminescent emission kinetics and spectra of mutated aequorins are included.

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Optimization of the DNA Purification Protocol for the Thermo Scientific KingFisher Flex and BindIt 3.1 Software
Application Note

Purpose: Optimal quantity and quality of DNA with the magnetic particle based extraction technology. Method: Thermo Scientific KingFisher Flex magnetic particle processor and BindIt® 3.1 Software. Results: By simple optimization of the nucleic acid extraction protocol it is possible to achieve a great yield of high quality DNA.

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