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Latest Application Notes

Application Note

The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH

ELISAONE ™ technology has been developed by TGR BioSciences to provide a means of running high performance sandwich immunoassays in a user-friendly 96-well format.
Application Note

Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules

During manufacture of antibody molecules, they are subjected to mechanical stress generated by processes such as pumping and filtration. This may cause denaturation and consequently aggregation due to exposure of the protein to air-liquid interfaces and shear forces, resulting in the ultimate loss in bioactivity.
Application Note

An AlphaScreen® SureFire® Phospho-ERK1/2 Assay Performed on BMG LABTECH’s POLARstar Omega

G-protein coupled receptors (GPCRs) are a large family of receptors which have a broad involvement in cellular responses affecting many important body functions both in health and disease.
Application Note

A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition

Pgp expression has been linked to the effl ux of chemotherapeutic
drugs in human cancers leading to multidrug resistance. Pgp
activity can also result in low oral absorption and poor brain
penetration. Interaction of drugs with the Pgp may also cause
an increase in toxicity of co-administered compounds.
Application Note

Pathway independent cell-based assays on the EnSpire Multimode Plate Reader with Epic® label-free technology

This application note details how the PerkinElmer EnSpire® Multimode Plate Reader with Corning® Epic® label-free technology can be used in multi-well microplate formats to non-invasively identify and characterize multiple G protein-coupled receptor (GPCR) pathways in living cells using an orthogonal approach.
Application Note

Comparison of Performance: EnSpire Multimode Plate Reader and Corning® Epic® System

In recent years, label-free technology has gained momentum as an innovative tool in the fields of cellular research and biochemical binding assays. This application note demonstrates the comparable performance of the EnSpire Multimode Plate Reader to the Corning® Epic® System.
Application Note

Quantitative Determination of Caffeine in Beverages and soft Drinks Using UV Wavelength Spectroscopy

Numerous studies, employing a wide variety of methods, have been carried out to determine the typical caffeine content of commonly consumed beverages. This application note details how UV spectroscopy can be used to analyse and quantify the caffeine content of some common beverages and soda drinks.
Application Note

Quantitative Determination of the Aspirin Content of Tablets Using UV and Visible Wavelength Spectroscopy

The aspirin content of commercial tablets is routinely determined using the acid/base titration method. This application note details the UV and Visible spectrophotometric methods for determining the aspirin content of tablets.
Application Note

Quantitative Determination of Glucose in an Energy Drink, Apple Juice and Wine

The use of the Glucose (HK) Assay Kit from Sigma can be used to measure the amount of glucose in three different types of drink.
Application Note

Use of the Microcuvette Holder with Plastic Microcuvettes

Common applications of UV/Visible spectrophotometers include direct measurement of nucleic acids and proteins to determine their concentration. Using Jenway UV plastic cuvettes with a microcuvette holder, these measurements are possible with as little as 70µl of sample.
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