Latest App Notes & Case Studies
App Note / Case Study
Efficient washing of magnetic beads
The use of magnetic beads as the solid phase in a multiplexed ELISA enables high throughput cytokine screening and offers several advantages over common ELISA assays with direct coupling of the analyte to the well-surface.
App Note / Case Study
Determination of microbial cell viability
The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
App Note / Case Study
Stem cell research: Creating blood for high throughput screening
Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
App Note / Case Study
Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences
One of the challenges in life sciences research today is to discover methods for running key assays more quickly, more reliably and using lower volumes of reagents and sample, but still with improved sensitivity. One area where this particularly holds true is for traditional enzyme-linked immunosorbent assays (ELISAs), whose application provides a useful measurement of antigens, including cytokines and a host of other biomarkers.
App Note / Case Study
Protein Purification Workflow Development Using Bio-Rad's NCO' Chromatography System
High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. This is, however, not always a viable option. This application note discusses protein purification workflow development for untagged proteins and introduce a new indicator of method performance, the purity quotient difference.
App Note / Case Study
Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.
App Note / Case Study
Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
App Note / Case Study
Trends, Challenges and Strategy in the Forensic Science Sector
The forensic science sector is in transition. New insights, technologies, and customers, combined with falling costs and increasing capabilities cause the sector to grow rapidly. As a consequence the role of forensic laboratories is changing.
App Note / Case Study
The Analysis of Common Drugs of Abuse in an Oral Fluid Matrix using a New Q TRAP® Platform
The multiplexing analysis of several compound classes, such as common prescription drugs along with drugs of abuse is very important. The measurement of these drugs has generally been performed using urine as the sample matrix. However, many research laboratories are finding the need to analyze these compounds in oral fluid matrices.
App Note / Case Study
Quantification of Ethylglucuronide in hair by Liquid Chromatography Tandem Mass Spectrometry
An advantage of the use of ethylglucuronide (ETG) in hair is that it is not subject to the effect of bacteria. It also offers the opportunity to extend the window of detection up to 6 months compared to hours- days in blood and urine.
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