Latest App Notes & Case Studies

ETD is a powerful fragmentation technique that has proved particularly useful for the determination of labile post-translational modifications of peptides and proteins. The technique has been used to determine the site-specific octanoylation of the peptide Ghrelin which could not be determined using Collision Induced Dissociation.

Post-translational histone modifications like acetylation play a pivotal role in the epigenetic regulation of transcription. Catalyzing the latter reaction HDACs affect various cellular processes especially cancerogenesis. Although the mechanism of starting cancerogenesis by epigenetic events is not clearly explained inhibition of HDACs has highlighted as a viable principle in cancer therapy.

The color of food has a great impact on consumer’s ultimate perception of food’s quality and flavor. Color additives are used for decorative purposes, enhance product identity, minimize variations in natural color and provide color stability on the shelf. Dye content is a tightly-controlled food-product characteristic with liquid chromatography being a widely accepted QA/QC technique for its determination.

While carbohydrate determination of most foodstuffs requires only minimal sample preparation such as dilution and filtration, samples with interfering matrices such as protein-containing dairy products have to be dialysed before injection. This application note describes a straightforward ion chromatographic method to sensitively determine water-soluble polyols and sugar alcohols as well as mono-, di- and oligosaccharides.

Here we demonstrate the detection of GLP-1R binding through the incorporation of a homogeneous cell-based binding assay using HTRF Tag-lite® technology.

The oxygen radical absorbance capacity (ORAC) assay has emerged as a robust analytical method to determine the antioxidant potential of a range of substances found in neutraceutical, pharmaceutical and food products. This application note demonstrates the ability to perform the assay in a 96-well microplate format amenable to higher throughput platforms.

This application note describes the configuration and performance of the Agilent 7890A GC system when using this method for the analysis of B100 biodiesel derived from algae oil.

Agilent Technologies DP technology is the detector of choice for sulfur analysis when dealing with a hydrocarbon matrix.

The 7696A Workbench is a standalone instrument specifically designed to automate sample preparation.

The enantiomers of linalool and other important components of natural oils or natural extracts including cypress oil, geranium extract, balm leaves oil, and jasmin oil are separated on a multidimensional system using two separate GC ovens.