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Latest App Notes & Case Studies

App Note / Case Study

Determining Total Sugar Content in Maple Syrup to Meet FDA Nutrition Labeling Requirements

Nutrition labels on food items provide valuable information to consumers. To ensure authenticity, the Food and Drug Administration checks the reported values on the nutrition labels. Due to advances in chromatography and universal analysis of sugars by refractive index detectors (RI), RI is the preferred method for sugar analysis. A total sugar analysis method for commercially available maple syrup was performed using Agilent HiPlex Ca Column and Agilent 1260 Infinity RI Detector.
App Note / Case Study

Analysis of Acharicides in Honey

To prevent economic losses beekeepers treat their colonies with acaricides. The use of these acaricides inside beehives implies a risk of direct pollution of honey and other hive products, therefore maximum residue levels on honey have been fixed in many countries to protect consumers.
App Note / Case Study

Two-Dimensional HPLC Combined with On-Line SPE for Determination of Sudan Dyes I–IV in Chili Oil

Although use of Sudan dyes in food is restricted, they are nevertheless sometimes added to foods to improve the appearance and command a higher price. Because Sudan dyes may create health problems such as genotoxic and carcinogenic effects, concerns over contamination of Sudan dyes has increased awareness and testing for these compounds.
App Note / Case Study

Fast Determination of Anthocyanins in Pomegranate Juice

Due to their strong antioxidant properties, anthocyanins are of considerable interest. Pomegranate juice (PJ) has been reported to contain 3× more antioxidant activity than green tea. Due to the high demand for pomegranates, adulteration of PJ has become widespread. Manufacturers have attempted to extend the limited supply by blending with filler ingredients such as cane sugar, corn syrup sweeteners, and lower-quality juices containing sorbitol, malic acid, and sucrose.
App Note / Case Study

Analysis of Fruit Juice Adulterated with Medium Invert Sugar from Beets

Fruit juice adulteration presents an economic and regulatory problem. The United States orange juice industry estimates that orange juice sales gross more than one billion dollars annually. The most common forms of adulteration include simple dilution and blending of inexpensive and synthetically produced juices into the more expensive ones.
App Note / Case Study

Kv1.5 Potassium Channel Assay Using the IonWorks HT System

This application note describes the use of the IonWorks HT Platform with a voltagedependent potassium channel Kv1.5.
App Note / Case Study

Contaminant bovine IgG assay

Fetal calf serum containing bovine IgG is commonly used in mammalian cell culture media for the production of recombinant pharmaceuticals. During the purification process of a recombinant pharmaceutical, bovine IgG may be copurified with the product, and may represent a biological hazard for clinical use. Therefore, it may be necessary to assay for contaminant bovine IgG in a final product originating from mammalian cells.
App Note / Case Study

Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System

Accurate measurement of the concentration of a biological product is important in bioreactor/fermenter monitoring, downstream processing and final product quality control. Currently, electrophoretic, chromatographic and immunochemical methods are the most commonly used. These three methods are currently used in combination to provide the desired sample information.
App Note / Case Study

Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers

Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.
App Note / Case Study

Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers

The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
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