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Acheiving Faster Ramp Rates for Thermal Experiments

Acheiving Faster Ramp Rates for Thermal Experiments content piece image

Hydrogen bonds between base pairs in nucleic acids can be broken at certain temperatures, allowing double stranded nucleic acids to be separated into single strands. A thermal melt experiment exploits the difference in the number of hydrogen bonds between DNA nucleotides, however the slow temperature ramp rate means that experiments take a long time.

The Agilent Cary 3500 Multizone UV-Vis spectrophotometer accurately controls the temperature of solutions during the experiment.

Download this application note to find out more about:

  • Discover how you can achieve faster ramp rates
  • Learn how to improve the productivity of your thermal melt experiments