Attamole Detection of Proteins in a Complex Mixture Using the SYNAPT G2-S System
Application Note Oct 05, 2012
Washington University School of Medicine
Protein identification is often challenged by the sensitivity and specificity required. For example, the presence of contaminating peptides within the collision cell during the collision-induced dissociation process leading to mixed fragment ion spectra is often ignored. This is overcome by the use of mobility assisted data independent acquisition (HDMSE) to qualitatively and quantitatively characterize enzymatic protein digests. This method has proven to be highly efficient when dealing with protein mixtures of varying complexity. Advancements in instrument technology allow HDMSE data acquisitions to be accompanied with increased sensitivity by incorporating StepWave™ ion optics. The StepWave device allows significantly more ions to be introduced while utilizing a robust mechanism for the elimination of neutral components and gas stream from the instrument, resulting in sub-femtomole sensitivity over a wide dynamic range. Here, we demonstrate the identification of known standard proteins spiked at varying levels into a complex sample matrix.