Automated Liquid-liquid Extraction of 25-Hydroxy Vitamin D from Serum and Preparation for LC/MC Analysis Using the Hamilton Robotics Microlab STARlet Workstation

Vitamin D is essential for the growth of healthy bone and its deficiency is often associated with several cardiovascular and autoimmune disorders in addition to depression, osteoarthritis, osteoporosis, cancer and many other diseases. There are several methods available to measure Vitamin D from blood serum samples, of which LC/MS is the most widely accepted in clinical labs. This application note describes the steps involved for automated extraction of 25-Hydroxy Vitamin D from blood serum and sample preparation forLC/MS analysis. An automated approach to this application eliminates tedious manual processes, resulting in an estimated 600% increase in processing and analysis efficiency.