Best Practices for In Vitro Transcription mRNA Analysis
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Reliable and robust quality control (QC) analysis is crucial throughout in vitro transcription (IVT) mRNA workflows, particularly in vaccine development and therapeutics.
Key QC steps include assessing the quality and size of the linearized plasmid, evaluating the size and purity of the IVT mRNA, measuring the length of the poly(A) tail and verifying the integrity of the final IVT mRNA vaccine product.
This application note outlines best practices for handling and analyzing IVT mRNA, ensuring accurate assessments of mRNA sizing, resolution and percent purity.
Download this application note to explore:
- The essential role of QC in mRNA vaccine development and therapeutics
- The importance of assessing mRNA quality, purity and sizing
- Best practices for accurate and reliable mRNA analysis during process development and production
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