Fast Determination of Anthocyanins in Pomegranate Juice

The method described in this application note is a sensitive, fast, and accurate way to determine anthocyanins in commercially available fruit juices using a simple dilution. Anthocyanins were separated using a 2.2 μm, Acclaim® RSLC 120, C18 rapid separation liquid chromatography column and detected at a visible wavelength of 540 nm. The silica-based column used in this application is designed for rapid, high-resolution separations, which is compatible with ultrahigh pressure instrumentation. The six anthocyanins of interest were separated in <5 min in various beverages that included PJ, grape juice, simulated adulterated PJ, pomegranate cherry juice, and pomegranate wildberry juice.