HPLC Analysis of Ashwagandha Supplements Using Alliance™ iS HPLC System
Credit: iStock
This study developed a High-Performance Liquid Chromatography (HPLC) method for the analysis of major withanolides in ashwagandha root and commercially available supplements. System suitability testing confirmed the method's performance. Ultrasound-assisted extraction with a 60:30:10 ethanol:methanol:water solvent for 30 minutes yielded the highest withanolide recovery from ashwagandha root. The method successfully separated and quantified the major withanolides in the USP reference root sample. However, analysis of commercially available ashwagandha supplements revealed the presence of only some of these withanolides, often in much lower quantities compared to the USP root sample.
