Improving qPCR Reliability With Automated RNA Normalization and Plate Setup
PIPETMAX®: Improving qPCR Reliability With Automated RNA Normalization and Plate Setup
Quantitative real time PCR, often coupled with reverse transcription (RT-qPCR), is a powerful technique for detecting differences in the copy number of nucleic acids and can be used for analysis of gene expression, copy number variation, detection of viruses, food safety testing and more.
To enable replication of experiments between labs, it is recommended to normalize the amount of input RNA used for reverse transcription, however, normalizing nucleic acid concentrations requires precise calculations and repetitive pipetting, which can be tedious and error-prone.
Download this app note to discover automation solutions that can:
- Normalize RNA concentrations before RT-qPCR while maintaining RNA integrity
- Guide users through experimental parameters including selection of controls, dilution factors and replicates
- Automate routine pipetting