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Measuring Lactose in Milk: A Validated Method

App Note / Case Study

Published: July 25, 2012

This application update describes a simple, rapid, and accurate method for the determination of lactose in milk using standard high-performance liquid chromatography with a Thermo Scientific Dionex Corona™ Charged Aerosol Detector (CAD™). The determination involves a simple 1:100 dilution of milk and high-performance liquid chromatography determination in 8 min. The method is validated with respect to specificity, linearity, accuracy, precision, and sensitivity.