Optimized Protocol for ATCC Assay Ready THP-1 Monocyte Differentiation With PMA
This protocol outlines the differentiation of ATCC Assay Ready THP-1 monocytes (ATCC® TIB-202-AR™) into macrophage-like cells using PMA, eliminating the need for prior cell culturing. It provides an optimized method for consistent macrophage differentiation, emphasizing aseptic techniques and immediate post-thaw seeding. The protocol will can be used to obtain better differentiation into macrophage-like cells with high efficiency and consistency.