Rapid point of use DNAble® assay for citrus greening disease (Candidatus Liberibacter spp.) using the Douglas Scientific® AmpliFire®

The AmpliFire by Douglas Scientific along with DNAble isothermal amplification chemistry from EnviroLogix® provide a simple and portable tool to perform genetic analysis at the point of use. The highly specific and accurate DNAble chemistry resolves past challenges for isothermal DNA amplification such as noisy background, interference from inhibitors, and false positives. This paper describes a proof-of-concept experiment that demonstrates the performance characteristics of a DNAble assay performed on the AmpliFire as compared to a real-time PCR instrument.

• The AmpliFire is a portable point of use detection instrument optimized for DNAble isothermal nucleic acid amplification.

• DNAble is a rapid and robust isothermal DNA amplification chemistry using a fluorescent-labeled molecular beacon for detection.