Running Assay and Impurity Analysis in One LC Method

Preclinical evaluation of monoclonal antibodies routinely relies on separate ligand binding assays and liquid chromatography mass spectrometry workflows to assess drug survival in vivo. A hybrid immuno-mass spectrometry protocol allows a laboratory to combine molecular capture and high-resolution detection into a single assay. This application note documents how a streamlined enrichment step reduces sample preparation time to 4–5 hours while maintaining a lower limit of quantification of 20 ng/mL from a 50 µL serum sample. Download this application note to see how: Streamlined bead-based extraction compresses traditional multi-day workflows into less than half a working day Selective target capture isolates humanized therapeutic proteins cleanly from complex animal serum matrices High-resolution accurate-mass analysis tracks intact structural variations without sacrificing quantitative sensitivity