Water in Mineral Oil in Water (W-O-W) Double Emulsion Production using SDS, PGPR and Tween® 80 as Emulsifiers

This application note reports on the production of monodisperse double emulsion droplets produced in a single step using a microfluidic approach. Double emulsions are engineered fluids consisting of one or more micrometer sized liquid emulsion droplets containing still smaller liquid droplets encapsulated within. At least two immiscible fluids are used in order for a liquid-liquid interface to develop. Here, the inner phase is water with SDS, the middle phase is mineral oil with PGPR, and the outer phase is water with Tween-80.