Enhancing Downstream Bioprocesses with Raman Spectroscopy

The accurate measurement of protein and excipient concentration in biomanufacturing processes is essential to production of therapeutics like monoclonal antibodies (mAbs), nucleic acids and other important drug products. Raman spectroscopy, a Process Analytical Technology (PAT) based on specific molecular signatures, is particularly suitable for monitoring complex biological processes characterized by multi-component interactions and dynamic changes.

In this compendium, we present a collection of studies demonstrating the application of Raman spectroscopy towards monitoring downstream bioproduction processes. When deployed as a PAT, Raman spectroscopy provides both qualitative and quantitative feedback, in real time, of proteins, amino acids, and even buffer quality. As the application notes contained herein show, Raman spectroscopy’s intrinsic capabilities allow it to be highly useful in bioprocess monitoring even when the presence of complex biomolecules or interferents like clarified harvest might render other analytical methods ineffective