A Balanced Subcellular Distribution of the Plant Immune Receptor Rx Between the Cytoplasm and Nucleus is Required for Effective Immunity Against Potato Virus X
Conference Recording Jan 20, 2013
About the Speaker
Wladimir Tameling received his PhD (2005) in Molecular Plant Pathology at the University of Amsterdam, The Netherlands. His research interest is to unravel the molecular mechanism of the plant’s innate immune system.
Cereal plants are an indispensible resource for food, feed, industrial raw material and energy. In addition to abiotic stress factors, fungal diseases are a major cause of yield losses worldwide. Since classical breeding is very unlikely to keep step with future requirements of crop improvement, the development of new technologies is necessary. We here report on the utilization of different methods to facilitate studies on molecular mechanisms of plant-fungal interactions. As a technical basis for the directed modulation of candidate genes, it contains a GATEWAY-compatible binary vector set for both overexpression and RNAi carrying promoters active in cereals. Further on, a transient-induced gene silencing (TIGS) system was developed for barley epidermis which has become instrumental to efficiently pre-screen large numbers of genes with regard to their effects in the context of the barley-powdery mildew pathosystem. To eventually validate the function of selected genes in stably transgenic plants, reliable protocols for Agrobacterium-mediated transformation were established for barley, wheat, rye, maize and triticale. Examples will be given that either enhance resistance or susceptibility of barley against the powdery mildew fungus.