Comprehensive microRNA Expression Profiling in Lineage Specific Human Hematopoiesis
Conference Recording Jun 18, 2013
About the Speaker
Nalini Raghavachari received her Ph.D in Biochemistry from the University of Madras, India. She has had post doctoral trainings in the field of genomics at Plant Genetic Engineering Laboratory in New Mexico, Baylor College of Medicine and University of Nebraska –Lincoln. Afterwards she worked as a drug development Scientist in Procertus Biopharma in Madison-Wisconsin followed by a Senior Research Scientist position in Corning Life Sciences division developing genomic tools for biomarker discovery. She joined NIH in 2003 and currently she is the co-director of the DNA Sequencing and Genomics Core Facility in NHLBI. Abstract
MicroRNAs constitute a recently discovered class of non-coding RNAs that play key roles in the regulation of gene expression. Acting at the post-transcriptional level, these fascinating molecules is believed to fine-tune the expression of as much as 30% of all mammalian protein-encoding genes. In human hematopoiesis, that requires a tight control of gene expression, it would be highly beneficial to elucidate the regulatory role of miRNAs in lineage specific gene expression for identifying novel drug targets. We performed microarray based analysis of miRNA expression during lineage specific differentiation of CD34+ cells. Analysis revealed 49 miRNAs with functional roles in cellular growth and proliferation, apoptosis to be significantly modulated during lineage specific differentiation. An integrated analysis of the differentially expressed miRNA and mRNA corroborated with the predicted targets by showing inverse correlation in the expression levels of several mRNA and miRNA that have functional role in hematopoietic process. We believe that this study would enhance our understanding of the regulatory role of miRNA in lineage specific gene expression.