Microchip Electrophoresis: On-Animal Sensors and Single Cell Analysis
Conference Recording Jan 01, 2014
About the SpeakerSusan M. Lunte is the Ralph N. Adams Distinguished Professor of Chemistry and Pharmaceutical Chemistry and Director of the Adams Institute for Bioanalytical Chemistry at the University of Kansas, Lawrence, KS. She is currently the Associate Editor for the Americas for Analytical Methods. Her research interests include the development of microfluidic based methodologies for separation and detection of peptides, amino acids, neuro¬trans¬mitters and pharmaceuticals in biological fluids.
Microchip electrophoresis has several advantages for monitoring of biological processes both in vivo and in vitro. These include the ability to analyze submicroliter samples, the potential to perform very fast highly efficient separations and the possibility of integrating the sample preparation and detection components of the analytical system directly into the chip. In this presentation, two examples of the use of microchip electrophoresis for neurochemical investigations will be presented. The first involves the development of a separation-based sensor for near real-time continuous monitoring of neurotransmitters and nitric oxide metabolites in awake freely roaming animals. The ultimate goal is to miniaturize the entire system for on-animal analysis using telemetry control for data acquisition. The second application involves the development of an analytical method for the detection of reactive oxygen and nitrogen species in single cells. Microchip electrophoresis with electrochemical and laser induced fluorescence detection is currently being employed to investigate RNOS generation in Jurkat cells and macrophages. The ultimate goal is to develop an analytical system for the detection of the production of intracellular nitric oxide and peroxynitrite present in single macrophages and microglia.