Microfluidics for Engineering 3D Tissues and Cellular Microenvironments
Conference Recording Jun 21, 2013
About the SpeakerBrian Gillette is a Postdoctoral Research Fellow in Sam Sia’s lab in the Department of Biomedical Engineering at Columbia University. He received his B.S. and Ph.D. in Biomedical Engineering at Columbia University. His doctoral work focused on developing microfabrication techniques for collagen-based tissue scaffolds for controlling in vitro cellular environments and for constructing engineered tissues for implantation. His current research interests involve applying microfabrication and microfluidics techniques for the development of vascularized skin and adipose tissues using adult stem cells and IPS cells.
Cellular microenvironments in native tissues are three dimensional (3D), inhomogeneous, anisotropic, and dynamic in terms of their composition of cells, extracellular matrix components, soluble factors, and physical forces (e.g. fluid flow and mechanical stress). Therefore, methods to recapitulate and control various components of cellular microenvironments in vitro and in vivo are highly useful for both studying and engineering biological systems. Microfluidics-based technologies enable precision spatiotemporal control over mass transport and are thus well suited to control the assembly and dynamic culture of engineered cellular microenvironments. In this presentation, I will highlight several different ways that microfluidics can be utilized in engineering 3D tissues and cellular microenvironments, including methods to microfabricate 3D tissue scaffolds using microfluidics, methods to assemble and dynamically culture 3D microenvironments within PDMS-based microfluidic devices, and methods to incorporate microfluidic channels directly within engineered 3D tissue scaffolds, for both perfusion of tissue constructs with media and for assembly of multiphase 3D tissues.