Dr. Sven Bocklandt profile page

Species Director

at Colossal Biosciences

Dr. Sven Bocklandt is Species Director at Colossal Biosciences, a bioscience company using genetic engineering to restore extinct animal species, protect critically endangered species and repopulate critical ecosystems. A human geneticist by training, he spent 10 years in technical leadership positions in genome analysis companies, and developed the epigenetic clock.

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Published Content

Total: 1

Webinar

Reviving the Past, Safeguarding the Future: Challenges and Innovations in Cell Culture for De-Extinction

Colossal Biosciences is bringing back extinct animal species through genetic engineering, and using advanced biotechnology methods to help preserve endangered species. This process requires a large number of precision genome edits to be made in single cells that can be isolated, grown monoclonally, expanded, and re-edited. While Colossal generates mouse models to test some of these edits, typically the cell lines cultured are non-model organisms that are poorly characterized and require extensive optimization.

In this webinar we will discuss the challenges picking gene edited mouse embryonic stem cell colonies, and difficulties isolating monoclonal cell lines from non-model organisms. We will discuss automated alternatives to flow-sorting and manual colony picking with that improve single-cell outgrowth and increase gene editing efficiency.