We will cover sample preparation, LC/MS data acquisition and data analysis methods for easily capturing the biological fingerprints of a variety of sample types. Emphasis will be given to hardware workflow solutions that can easily be transferred to a new lab and that utilize intuitive software tools.

This talk will cover two complementary technologies for measuring metabolic flux: Seahorse XF analyzers and liquid chromatography-mass spectrometry (LC/MS) qualitative flux analysis. Both technologies provide important insights into the rates of metabolic reactions within cells, with Seahorse XF measurements providing cellular resolution, and LC/MS qualitative flux measurements providing molecular resolution.