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Development and Validation of a Duplex qPCR Assay for the Detection of Residual Sf9 Host Cell DNA and Baculovirus DNA content piece image
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Development and Validation of a Duplex qPCR Assay for the Detection of Residual Sf9 Host Cell DNA and Baculovirus DNA

Current regulatory authorities (WHO, EMA and US FDA) limited the accepted amounts of residual DNA in biological products making it extremely important to have a sensitive method of quantifying residual host cell DNA. Among the methods of detecting residual DNA, qPCR is the most widely used for residual DNA quantitation due to its sensitivity, accuracy, precision, and time-saving capability.
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