Embryonic Hand of a Madagascar Giant Day Gecko (Phelsuma grandis)
This image of an embryonic Madagascar giant day gecko (Phelsuma grandis) hand was created by Grigorii Timin, with supervision from Dr. Michel Milinkovitch, using high-resolution microscopy and image-stitching. The nerves are shown in a cyan color and the bones, tendons, ligaments, skin and blood cells are shown in a range of warmer colors.
The image placed first in the Nikon Small World 2022 Photomicrography Competition.
To prepare the sample, Timin performed whole-mount fluorescent staining and tissue clearing to capture the entire embryonic hand with a confocal microscope. “This embryonic hand is about 3 mm (0.12 in) in length, which is a huge sample for high-resolution microscopy,” said Timin. “The scan consists of 300 tiles, each containing about 250 optical sections, resulting in more than two days of acquisition and approximately 200 GB of data.”