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App Note

Mass-Based Purification of Natural Product Impurities

Agilent--Product1260-LP-May.2
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Agilent--Product1260-LP-May.2

Organic impurities can be more than a minor inconvenience in the making of high‑quality products such as food additives or dietary supplements.

Preparative‑scale High Performance Liquid Chromatography (HPLC) is frequently applied to separate and isolate unknown by-products for further characterization.

Download this application note to discover:

  • A workflow from analytical scouting to preparative purification
  • An analysis of a natural product extract with two unknown impurities
  • How to achieve linear scale-up to preparative conditions with reproducible separation of the target compounds
  • How to achieve highly specific collection that yields pure fractions suitable for further analyses
   

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