DNA methylation plays an important role in cell growth by regulating gene expression. In some cases, aberrant cytosine methylation can lead to carcinogenic changes in gene expression. Historically, these modifications have been hard to identify in patient samples. Although next-generation sequencing (NGS) of DNA treated with sodium bisulfite has provided an efficient means for the detection of methylated cytosines, the chemical conversion process is damaging, leading to GC biased coverage and poor complexity.
