Posttranslational modifications (PTMs) are covalent processing events that change the properties of a protein by addition of a modifying group to one or more of its amino acids. Due to its prominent role in cellular signaling, protein phosphorylation is one of the most studied PTMs.
Mass spectrometry-based phosphoproteomics now routinely measures tens of thousands of phosphorylation sites on a proteome-wide scale. Increasing the throughput and sensitivity of phosphoproteomics approaches is highly desirable to disentangle their functional relevance.
Download this app note to discover a platform that can:
- Identify more than 12,500 phosphopeptides from low input material without the need for a project-specific library
- Detect more than 9,300 phosphopeptides, including co-eluted position isomers, in short 7-minute gradients
- Produce results with a high degree of reproducibility and data completeness
