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Latest How To Guides

8 Steps to Successful SMRT Sequencing
How To Guide

When Single Molecule, Real-Time (SMRT) sequencing was first released, it promised a huge shift in our approach to sequencing – moving away from methods that had up to that point been validated and entrenched in labs across the world. In this guide, PacBio's Nick Sisneros provides some insight into SMRT Sequencing and the tips and tricks that will ensure success in the template prep and sample preparation for use on this sequencing platform.

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8 Tips for Improving Your NGS Libraries
How To Guide

For most laboratory workflows, you’re only as good as your starting material. This is particularly true for next-generation sequencing (NGS). Here are several tips and tricks to preparing NGS libraries that hold true across all applications.

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6 Essential Lab Safety Rules
How To Guide

Download this guide to explore the essential guidelines that every laboratory and scientist should adhere to when handling sensitive or delicate samples.

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How To Make Neurons: Differentiating pluripotent stem cells to cortical or hypothalamic neurons
How To Guide

This guide outlines protocols for the differentiation of hPSCs to either cortical neurons or hypothalamic neurons, provides key pointers for researchers who are undertaking these protocols for the first time.

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How To Guide: Field Spectroscopy
How To Guide

Field spectroscopy enables scientists to measure how different materials interact with electromagnetic energy and therefore underpins the technology enabling us to view the world from the air or space. Here we explore some tips for perfecting your field spectroscopy skills.

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4 Tips for Successful Cryopreservation
How To Guide

This list covers 4 aspects of cryopreservation to consider when preserving your precious samples.

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5 Steps to Successful Liquid Chromatography
How To Guide

Download this guide to discover:
- How to set up you HPLC for successful results
- How to troubleshoot common HPLC-related issues
- A step-by-step guide to using your HPLC and gaining meaningful data

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5 Key Steps to Successful CRISPRi
How To Guide

The CRISPR interference (CRISPRi) machinery is an efficient, reversible and specific genome regulating platform for transcription control, without altering the target DNA sequence. This guide includes key steps on implementing CRISPRi using dCas9 derived from Streptococcus pyogenes.

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10 Tips for Successful Development of Cell Culture Assays
How To Guide

While technological advances are reshaping the cell culture field, many basic steps are still to this day performed manually in laboratories. This guide highlights 10 tips for successfully developing cell culture assays.

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7 Tips for Successful Development of 3D Cell Culture Assays
How To Guide

While the shift to 3D cell culture has been under way for the last decade, it is still evolving rapidly to meet the ever growing and dynamic pharmaceutical demand.
Here we provide tips which we deem essential for anyone carrying out 3D cell culture.

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