7 Tips to Improve Your Western Blot
How To Guide Jun 22, 2018 | by Karishma Joshi, 2nd Year PhD Student, Department of Neuroscience, Psychology & Behaviour, The University of Leicester, UK
How hard can it be to get a nice single band on a clean blot? The answer, VERY!
Follow these 7 top tips to improve your westerns and reap the rewards of clean bands and publishable quality data, every time.
This how-to guide gives you practical advice on:
- Cleaning your equipment
- Making your best gel
- Setting up electrodes
- Transferring proteins from gel to membrane
- Choosing the right antibody
- Testing your antibodies
RELATED HOW TO GUIDES
Publishing your work in well-respected academic journals is essential to progressing your career in your chosen field. The sheer volume of journals that are available can be daunting and the process in which they operate can be mystifying. To help you better understand how this process works and to give you a basic overview of what to expect, we’ve broken it down into 5 key steps.
Like what you just read? You can find similar content on the communities below.Analysis & Separations Applied Sciences Biopharma Cancer Research Cell Science Diagnostics Drug Discovery Genomics Research Informatics Proteomics & Metabolomics Neuroscience Immunology & Microbiology
To personalize the content you see on Technology Networks homepage, Log In or Subscribe for FreeLOGIN SUBSCRIBE FOR FREE