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Mastering Fluorochrome Selection for Clearer Data

Scientist preparing samples for flow cytometric analysis in the laboratory
Credit: iStock

Hundreds of fluorochromes are available to users conducting flow cytometry assays, each with unique properties that influence panel resolution and data quality. Selecting the right combination can be overwhelming, leading many researchers to rely on familiar choices that may introduce spillover and reduce clarity.


It’s essential to optimize panel design to minimize data loss and improve resolution. This guide simplifies fluorochrome selection by ranking them based on spillover and resolution, helping users design cleaner, more effective panels.


Download this guide to discover:

  • How fluorochrome spillover impacts resolution and data interpretation
  • Strategies for selecting fluorochromes based on antigen density and expression
  • A performance chart to simplify panel design and enhance data quality
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