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Tips To Optimize Imaging With Antibodies

Antibodies are a cornerstone of numerous biological and biomedical research areas, having long been used for imaging protein distribution in tissue and cells. Applications range from more conventional techniques, such as tissue immunohistochemistry (IHC) and cell immunocytochemistry (ICC), to more recent methods, such as proximity ligation assays (PLA) and immuno-PCR (iPCR).

Although now largely routine, antibody-based visualization methods require careful consideration of multiple parameters to obtain high-quality images.

Download this how to guide for tips on optimizing imaging with antibodies, with details on:

  • Antibody selection
  • Sample processing
  • Enhancing signal-to-noise ratio
Meet the Author
Masha Savelieff, PhD
Masha Savelieff, PhD