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Choose the Right Reaction Chemistry for Real-Time PCR

Thermo Fisher Scientific infographic comparing SYBR Green I dye and TaqMan Assays for real-time PCR analysis.
Credit: Technology Networks

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. 


TaqMan assays and SYBR Green dye are two popular approaches, each offering distinct advantages depending on a lab’s needs. By understanding these distinctions, researchers can select the approach that best aligns with their experimental goals, ensuring efficiency and precision.


This infographic explores key differences to help researchers determine the most suitable approach for their experimental workflows.


Download this infographic to:

  • Explore differences in detection methods, assay components and signal generation
  • Understand the impact of assay specificity on target and nontarget detection
  • Compare workflow design and optimization for real-time PCR experiments

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