3D Biomatrix and Cayman Chemical Partner
News Nov 25, 2013
3D Biomatrix announced that they have collaborated with Cayman Chemical to develop and supply a new assay kit for measuring the viability of 3D spheroids. The kit, Perfecta3D® Cell Viability Kit, is one of the first viability assays optimized for 3D cell culture.
Researchers have long tested drug compounds and other factors affecting cell growth in two-dimensions (2D), but 2D cell cultures do not accurately represent how cells behave in the body. 3D cell cultures allow researchers to recapitulate these characteristics in vitro, providing a more physiologically-relevant model. 3D Biomatrix’s Perfecta3D Hanging Drop Plates facilitate the consistent and controllable growth of small spherical 3D clusters called spheroids in a well-plate format. Researchers can then test compounds and other factors in a 3D environment that reflects the human body.
The new Perfecta3D 3D Cell Viability Kit provides an easy-to-use format for studying cell proliferation in spheroids formed in the Hanging Drop Plates. The assay, based on the enzymatic cleavage of the tertrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases in viable cells, will allow investigators to screen drug candidates involved in cell cycle regulation in a 3D format.
“Our new 3D Cell Viability Assay Kit provides researchers with a solution for 3D cell culture and analysis,” said 3D Biomatrix CEO Laura Schrader. “As Cayman and 3D Biomatrix are leaders in their respective fields, researchers can be confident they are using reagents that have been specifically optimized for their needs in 3D cell culture.”
Jeff Johnson, Vice President of Biochemistry at Cayman Chemical says, “The 3D Biomatrix Perfecta3D Hanging Drop Plate is adding greater versatility to our growing line of cell-based assays. We are excited about the collaboration and the impact it will have on cell biology research.”
CRISPR Reveals New Targets for Promising Cancer DrugsNews
Novel screening method identifies new drug targets that could potentially enhance the effectiveness of PD-1 checkpoint inhibitors, a promising new class of cancer immunotherapy.READ MORE
Using Milk Protein to 3D-Imprint Muscle and Bone CellsNews
Researchers from the University of Canterbury are replicating a 3D imprint of cells onto films made of milk protein. The films then gradually degrade, leaving the grown tissue behind.READ MORE
Comments | 0 ADD COMMENT
EMBL Conference: European Conference of Life Science Funders and Foundations
Apr 19 - Apr 20, 2018
EMBL Course: Transgenic Animals - Micromanipulation Techniques
Apr 10 - Apr 11, 2018
EMBO Practical Course: Extracellular Vesicles: From Biology to Biomedical Applications
Apr 09 - Apr 13, 2018
EMBO | EMBL Symposium: Tissue Self-Organisation: Challenging the Systems
Mar 11 - Mar 14, 2018
EMBL Course: Target Engagement in Biology and Drug Discovery
Feb 19 - Feb 23, 2018