Central European Effort on High-Throughput Expression Profiling
News Jun 10, 2008
As part of major central European effort on high throughput real-time PCR expression profiling, the Institute of Biotechnology (IBT) of the Czech Academy of Sciences is acquiring a Fluidigm BIOMARK Genetic Engineering System.
While conventional real-time PCR instruments measure 96 reactions in parallel, the microfluidic BIOMARK platform can analyze up to 96 x 96 parallel reactions simultaneously.
“This exceedingly high-throughput will be extremely useful for our single cell expression profiling work” says Dr. Mikael Kubista, head of the laboratory of gene expression. A few years ago his team demonstrated that expression, measured on regular samples that contain thousands or more cells, does not reflect the situation among the individual cells.
“Measuring expression of 50-100 genes per cell, we can identify new cell subtypes and learn more about the mechanisms behind their responses to stimuli, environmental changes, drug treatment and also differentiation,” Dr. Kubista continues. “The Fluidigm BIOMARK system will also be very important for our work on multimarker diagnostics, disease monitoring, and prognostics of complex diseases."
“IBT is partner in several European, as well as National, projects related to expression profiling and sample management. We welcome new partnerships to which we can contribute with our cutting edge expertise, exceeding experience, and unique instrumentation in the molecular diagnostics field” says Dr. Peter Sebo, director of IBT.
IBT collaborates closely with TATAA Biocenter, a spin-off company from Kubista’s research group, on hands-on training on real-time PCR based applications.
The courses are supported by leading Biotech companies as well as MultiD Analyses, another of their spin-offs, which develops user friendly GenEx software for advanced real-time PCR expression profiling and multimarker analysis.
“The BIOMARK will be very important for our new course on high-throughput expression profiling and multimarker disease analysis”, says Henrik Sjostrom, head of TATAA Biocenter training organization. “It will make our popular Prague training site even more attractive.”