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DNA Moves Steadily During Interphase
News

DNA Moves Steadily During Interphase

DNA Moves Steadily During Interphase
News

DNA Moves Steadily During Interphase

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The human body is composed of over forty trillion cells. Each of these cells has totally two meters of tightly packaged genomic DNA, the blueprint of life. Recently, there have been many advances in understanding how DNA is packaged and organized as chromatin in the cell. In contrast, how chromatin behaves in living cells remains unclear.



Video 1: Movie data (50 ms/frame) of single nucleosomes (basic units of chromatin) fluorescently labeled in a living human cell. Note that clear, well-separated dots and their movements were visualized.


SOKENDAI graduate student Shiori Iida, JSPS Fellow Yuji Itoh, technical staff Sachiko Tamura, and Professor Kazuhiro Maeshima of the Genome Dynamics Laboratory (NIG), together with research scientist Soya Shinkai and team leader Shuichi Onami of RIKEN BDR, and Professor Masato T. Kanemaki of the Molecular Cell Engineering Laboratory (NIG), have investigated the local movements of chromatin in living human cells using super-resolution fluorescence microscopy.



Video 2: Movie data (50 ms/frame) of single nucleosomes fluorescently labeled in living human cells; (left) G1-phase, (right) G2-phase. Note that there is not much difference in nucleosome motion between G1 and G2 phase, even as nuclear size increases.


Both DNA amount and nuclear size become double during the preparation period for the cell division (interphase). Previously, it has been suggested that these drastic changes in the nuclear environment would affect chromatin movements. However, Iida et al. have revealed that chromatin motion keeps a steady state throughout the interphase. Chromatin motion is directly related to the accessibility of the DNA (readability of genomic information). Steady-state chromatin motion allows cells to conduct housekeeping tasks under similar environments during interphase.


Reference: Iida S, Shinkai S, Itoh Y, et al. Single-nucleosome imaging reveals steady-state motion of interphase chromatin in living human cells. Sci Adv. 2022;8(22):eabn5626. doi: 10.1126/sciadv.abn5626


This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.


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