Dolomite Bio’s Single Cell RNA-Seq System Enables High Speed Encapsulation
News Sep 02, 2016
This compact, scalable system enables rapid, reproducible droplet encapsulation of individual cells, allowing up to 10,000 single cell libraries to be generated in one 15-minute run.
The Single Cell RNA-Seq System allows up to 500 µl samples of cells and oligo-barcoded beads to be encapsulated in extremely monodisperse droplets, each containing a single cell. It incorporates all the components required to encapsulate individual cells, including the hardware – pumps, flow sensors, connectors and a high speed camera and microscope – advanced Flow Control Center software and accessories. The system uses a glass Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop¬seq chip described by Macosko et al1 – offering faster run time, longer life and greater ease of use. Installation and user training is included with each system, ensuring straightforward implementation and rapid initiation of droplet encapsulation experiments. Following encapsulation, the cell is lysed, allowing the mRNA to be captured on the beads for downstream recovery and sequencing.
In treating inflammatory bowel disease (IBD), physicians can have a hard time telling which newly diagnosed patients have a high risk of severe inflammation or what therapies will be most effective. Now researchers report finding an epigenetic signature in patient cells that appears to predict inflammation risk in a serious type of IBD called Crohn’s disease.