Dolomite Bio’s Single Cell RNA-Seq System Enables High Speed Encapsulation
News Sep 02, 2016
This compact, scalable system enables rapid, reproducible droplet encapsulation of individual cells, allowing up to 10,000 single cell libraries to be generated in one 15-minute run.
The Single Cell RNA-Seq System allows up to 500 µl samples of cells and oligo-barcoded beads to be encapsulated in extremely monodisperse droplets, each containing a single cell. It incorporates all the components required to encapsulate individual cells, including the hardware – pumps, flow sensors, connectors and a high speed camera and microscope – advanced Flow Control Center software and accessories. The system uses a glass Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop¬seq chip described by Macosko et al1 – offering faster run time, longer life and greater ease of use. Installation and user training is included with each system, ensuring straightforward implementation and rapid initiation of droplet encapsulation experiments. Following encapsulation, the cell is lysed, allowing the mRNA to be captured on the beads for downstream recovery and sequencing.
Bioethics Council Rules Heritable Genome Editing "Ethically Acceptable" In Certain CircumstancesNews
A leading UK bioethics advisory body has weighed in on the debate around human genetic modification, concluding that heritable genome editing – modifying the DNA of an egg, sperm or embryo with changes that will be passed on to future generations – could be ‘morally permissible’ in humans, provided key ethical tests are met.
Hay Fever Risk Genes Overlap with Autoimmune DiseaseNews
In a large international study involving almost 900,000 participants, researchers from the University of Copenhagen and COPSAC have found new risk genes for hay fever. It is the largest genetic study so far on this type of allergy, which affects millions of people around the world.READ MORE
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Scientists have long known that RNA encodes instructions to make proteins. In a new study published in Nature, scientists describe how the protein-making machinery identifies alternative initiation sites from which to start protein synthesis.READ MORE