Findings to Help in Design of Drugs against Virus Causing Childhood Illnesses
News Mar 22, 2013
The virus, called enterovirus 71, causes hand, foot and mouth disease and is common throughout the world. Although that disease usually is not fatal, the virus has been reported to cause fatal encephalitis in infants and young children, primarily in the Asia-Pacific region.
Currently, no cure exists for the infection.
New findings show the precise structure of the virus bound to a molecule that inhibits infection. The findings are detailed in a paper appearing this week in Proceedings of the National Academy of Sciences.
"These results provide a structural basis for development of drugs to fight enterovirus 71 infection," said Michael G. Rossmann, Purdue University's Hanley Distinguished Professor of Biological Sciences.
Rossmann is co-author of a paper with Purdue postdoctoral research associate Pavel Plevka; research scientist Rushika Perera; postdoctoral research associate Moh Lan Yap; Jane Cardosa, a researcher at Sentinext Therapeutics in Malaysia; and Richard J. Kuhn, a professor and head of Purdue's Department of Biological Sciences.
The researchers had previously used a technique called X-ray crystallography to determine the virus's precise structure. A small molecule called a "pocket factor" is located within a pocket of the virus's protective shell, called the capsid. When the virus binds to a human cell, the pocket factor is squeezed out of its pocket resulting in the destabilization of the virus particle, which then disintegrates and releases its genetic material to infect the cell and replicate.
Researchers led by Rossmann have developed antiviral drugs for other enteroviruses such as rhinoviruses that cause the common cold. The drugs work by replacing the pocket factor with a molecule that binds more tightly than the real pocket factor, inhibiting infection.
In the new work, the researchers obtained a near-atomic-scale resolution three-dimensional structure of enterovirus 71 binding with an inhibitor called WIN 51711.
"We show that the compound stabilizes the virus and limits its infectivity, probably through restricting dynamics of the capsid necessary for genome release," Rossmann said. "Our results provide a structural basis for development of antienterovirus 71 capsid-binding drugs."
At a resolution of 3.2 angstrom, the images show nearly atomic-scale structural features.
Hand, foot and mouth disease, an infection most common among young children, sometimes arises in a daycare setting. Of the 427,278 cases of the disease recorded in mainland China between January and May 2010, 5,454 cases were classified as severe, with 260 deaths, according to the World Health Organization.
The research was supported by the National Institutes of Health and the U.S. Department of Energy.
CRISPR Reveals New Targets for Promising Cancer DrugsNews
Novel screening method identifies new drug targets that could potentially enhance the effectiveness of PD-1 checkpoint inhibitors, a promising new class of cancer immunotherapy.READ MORE
BioAscent Announces New CEO and Plans to Expand Discovery Services OfferingNews
BioAscent Discovery Limited appoints new CEO, Paul Smith in preparation for the expansion of companies discovery services.READ MORE
Cell Recycling System Offers Therapeutic Entry Point for Rare Disease TreatmentNews
Scientists have demonstrated how an investigational drug works against a rare, fatal genetic disease, Niemann-Pick type C1 (NPC1).READ MORE
Comments | 0 ADD COMMENT
EMBL Conference: European Conference of Life Science Funders and Foundations
Apr 19 - Apr 20, 2018
EMBL Course: Target Engagement in Biology and Drug Discovery
Feb 19 - Feb 23, 2018
18th International Conference and Exhibition on Analytical & Bioanalytical Chromatographic Techniques
Nov 02 - Nov 03, 2017